chemoattractant il 8 (Kingfisher Biotech)
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chemoattractant il 8
![Age-specific changes in JAK2 regulation of neutrophil migration. (A) Functional heatmap of select pathways in secretome of neutrophils from young and aged mice across conditions (normalized to control). Heatmap show z -scores generated from P values and log fold/ratio changes. JAK inhibition (baricitinib [BAR], pan-JAK; AZD1480 [AZD], JAK2) increased regulation of IGF and chemokine signaling pathways compared to PMA treatment in neutrophils from young mice. In neutrophils from aged mice, both AZD and BAR treatment only increased regulation of IGF compared to PMA treatment. (B) Functional heatmap of migration-associated pathways in the cell fraction of samples. AZD treatment (JAK2 inhibition) led to an increase in integrin signaling, IL-1 signaling, and actin cytoskeleton signaling, and a decrease in actin nucleation by ARP-WASP compared to PMA treatment in neutrophils isolated from young mice. In neutrophils from aged mice, JAK2 inhibition increased all listed functions compared to PMA treatment, except for actin nucleation by ARP-WASP. BAR treatment (pan-JAK inhibition) led to an increase in integrin signaling and a decrease in the rest of the listed functions compared to PMA treatment in neutrophils from young mice. BAR-treated neutrophils from aged mice increased integrin signaling and decreased actin cytoskeleton signaling and actin nucleation by ARP-WASP, with no changes in IL-1 signaling compared to PMA-treated cells. (C) Heatmap depicting abundance of protein (log ratio normalized to control), associated with neutrophil motility. Overall, JAK regulation on neutrophil motility proteins is age dependent. AZD-treated neutrophils from young and aged mice showed a decrease in ARPC2 and an increase in myristoylated alanine-rich c-kinase substrate. AZD-treated neutrophils from aged mice had a decrease in I-κB kinase. BAR-treated neutrophils from young and aged mice showed increased VCAM1. BAR-treated neutrophils from young mice display decreased STAT3 and NF-κB. Those from aged mice showed a decrease in ARPC2 and I-κB kinase. (D) Bar graphs showing the relative abundance of 2 cytoskeletal proteins in each condition. ARPC2 and ARPC3 levels were decreased in neutrophils from young and aged mice in the presence of AZD (JAK2 inhibition). In neutrophils from young mice, BAR (pan-JAK inhibition) also decreased ARPC2 levels. (E) Proposed model of molecular mechanism of JAK2 modulation of neutrophil migration. We propose that JAK2 promotes the assembly of the actin nucleation complex by increasing the levels of ARPC2/3. This leads to actin branching and formation of pseudopodia to facilitate movement. Age-dependent regulation of migration by JAK2 occurs through its differential modulation of neutrophil chemokine release (increased CXCL2 in neutrophils from aged mice, and decreased SDF-1α in neutrophils from young mice). (F) Functional assessment of migration showed that overall, fewer neutrophils from age mice migrate <t>toward</t> <t>IL-8</t> compared to those from young mice. Only BAR significantly decreased neutrophil migration of neutrophils from young mice compared to PMA treatment. (G, H) Migration pattern between neutrophils from young and aged mice after pan-JAK or JAK2 inhibition (young: n =13/condition; aged: n =11/condition). * P <0.05; bar graphs represent mean±SEM. (D, F) Individual points correspond to individual mice (pink, females; green, males). Diagram generated with BioRender.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8130/pmc12908130/pmc12908130__nihms-2136105-f0002.jpg)
Chemoattractant Il 8, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chemoattractant+il+8/Human+IL-8+Recombinant+Protein/pmc12908130-56-21-25
Average 94 stars, based on 5 article reviews
Chemoattractant Il 8, supplied by Kingfisher Biotech, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chemoattractant+il+8/Human+IL-8+Recombinant+Protein/pmc12908130-56-21-25
Average 94 stars, based on 5 article reviews
chemoattractant il 8 - by Bioz Stars,
2026-11
94/100 stars
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1) Product Images from "Aging changes the mechanism that underlies JAK2 modulation of neutrophil function"
Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function
Journal: Journal of immunology (Baltimore, Md. : 1950)
doi: 10.1093/jimmun/vkaf323
Figure Legend Snippet: Age-specific changes in JAK2 regulation of neutrophil migration. (A) Functional heatmap of select pathways in secretome of neutrophils from young and aged mice across conditions (normalized to control). Heatmap show z -scores generated from P values and log fold/ratio changes. JAK inhibition (baricitinib [BAR], pan-JAK; AZD1480 [AZD], JAK2) increased regulation of IGF and chemokine signaling pathways compared to PMA treatment in neutrophils from young mice. In neutrophils from aged mice, both AZD and BAR treatment only increased regulation of IGF compared to PMA treatment. (B) Functional heatmap of migration-associated pathways in the cell fraction of samples. AZD treatment (JAK2 inhibition) led to an increase in integrin signaling, IL-1 signaling, and actin cytoskeleton signaling, and a decrease in actin nucleation by ARP-WASP compared to PMA treatment in neutrophils isolated from young mice. In neutrophils from aged mice, JAK2 inhibition increased all listed functions compared to PMA treatment, except for actin nucleation by ARP-WASP. BAR treatment (pan-JAK inhibition) led to an increase in integrin signaling and a decrease in the rest of the listed functions compared to PMA treatment in neutrophils from young mice. BAR-treated neutrophils from aged mice increased integrin signaling and decreased actin cytoskeleton signaling and actin nucleation by ARP-WASP, with no changes in IL-1 signaling compared to PMA-treated cells. (C) Heatmap depicting abundance of protein (log ratio normalized to control), associated with neutrophil motility. Overall, JAK regulation on neutrophil motility proteins is age dependent. AZD-treated neutrophils from young and aged mice showed a decrease in ARPC2 and an increase in myristoylated alanine-rich c-kinase substrate. AZD-treated neutrophils from aged mice had a decrease in I-κB kinase. BAR-treated neutrophils from young and aged mice showed increased VCAM1. BAR-treated neutrophils from young mice display decreased STAT3 and NF-κB. Those from aged mice showed a decrease in ARPC2 and I-κB kinase. (D) Bar graphs showing the relative abundance of 2 cytoskeletal proteins in each condition. ARPC2 and ARPC3 levels were decreased in neutrophils from young and aged mice in the presence of AZD (JAK2 inhibition). In neutrophils from young mice, BAR (pan-JAK inhibition) also decreased ARPC2 levels. (E) Proposed model of molecular mechanism of JAK2 modulation of neutrophil migration. We propose that JAK2 promotes the assembly of the actin nucleation complex by increasing the levels of ARPC2/3. This leads to actin branching and formation of pseudopodia to facilitate movement. Age-dependent regulation of migration by JAK2 occurs through its differential modulation of neutrophil chemokine release (increased CXCL2 in neutrophils from aged mice, and decreased SDF-1α in neutrophils from young mice). (F) Functional assessment of migration showed that overall, fewer neutrophils from age mice migrate toward IL-8 compared to those from young mice. Only BAR significantly decreased neutrophil migration of neutrophils from young mice compared to PMA treatment. (G, H) Migration pattern between neutrophils from young and aged mice after pan-JAK or JAK2 inhibition (young: n =13/condition; aged: n =11/condition). * P <0.05; bar graphs represent mean±SEM. (D, F) Individual points correspond to individual mice (pink, females; green, males). Diagram generated with BioRender.
Techniques Used: Migration, Functional Assay, Control, Generated, Inhibition, Protein-Protein interactions, Isolation
Related Articles
Migration:Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function. Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained Functional Assay:Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function. Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained Control:Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function. Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained Generated:Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function. Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained Inhibition:Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function. Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained Protein-Protein interactions:Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function. Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained Isolation:Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained Article Title: Aging changes the mechanism that underlies JAK2 modulation of neutrophil function. Article Snippet: Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).Boyden chamber insets, with 3-μm pores, were purchased from Abcam (catalog #ab235692).. These transwell chambers were placed on a 96-well plate; the lower chamber of the well contained |